Activated beads

Product No.: 1300

MAGicBeads ACT

Surface activated magnetic agarose beads employing our patented coupling chemistry. Any ligand containing amino groups or thiols, e.g. proteins and peptides can be covalently bound to the beads using a simple process. All steps are performed at physiological conditions and room temperature, without using any hazardous reagents or organic solvents. After coupling of the ligand, the magnetic beads can be used for affinity purification and immunoprecipitation.

• Activated magnetic beads for coupling your own ligands of choice
• Simple coupling process at ambient temperature
• Suitable for coupling of ligands containing sulphydryl- or amino groups
• Gives a stable bond between bead and ligand
• High coupling capacity, 10-30mg IgG/ml beads
• Couple 20mg IgG/ml beads with a yield of 90%

At the research lab, small-scale processing of peptides, proteins, antibodies or cells from crude sample mixtures like serum, whole blood, ascites, food-samples or unclarified cell cultures can be performed in volumes from 100 µl to 2 ml. By using 10-200 µl of MAGicBeads together with the MAGicSep LAB cube or LAB rack enables fast, reliable and easy separation of up to 6 mg of proteins per each tube via serial or parallel-process manual configuration.

Our MAGicBeads are also compatible with robotic system such as the KingFisher™ Flex unit (Thermo Fisher) allowing the use of our beads in automatic setups for high-throughput screening.

During the production phase of biopharmaceuticals, large-scale recovery of the product from an unclarified cell-culture is performed in a one step clarification and purification by using the propietary designed MAGicSep PROCESS units together with the affinity-coupled MAGicBeads. The magnetic filter separators are capable to capture up to 40 liters of MAGicBeads at high flow rates making them suitable to process up to kilo grams of proteins or antibodies per purification cycle.

By utilizing the magnetic-based capture approach, the biopharmaceutical can be recovered directly from the cell-containing culture with the same or better quality as for a conventional 2-step clarification and purification mode using depth filters and column chromatography.

• Process scale recovery of biologics
• High flow rates, 100-800 liter/h
• Pharmaceutical standard
• No column-clogging issues
• Automated
• 24/7 operation
• Captures 1-40 liters of MAGicBeads per cycle
• Process 10-4,000 liters of culture volumes
• Recover kgs of mAbs per cycle