OUr products

Magnetic resins and separators for scaleable recovery of biologics.

Magnetic resins
MAGic bioprocessing provides magnetic agarose-based resins that are used to recover biologics or other biomolecules directly from crude sample mixtures eliminating the need of centrifugation or filtration. Our MAGicBeads are highly porous which provides a large available surface area (50m2/ml) resulting in exceptional high binding capacities, similar to column resins. The beads are also strongly magnetized (40emu/g) and stable due to our proprietary manufacturing method which provides consistent and fast magnetic separation even in large sample volumes (litres).

Magnetic separators
MAGic bioprocessing provides magnetic separators (MAGicSep) that are optimized for easy and efficient capture of the magnetic resin from crude sample mixtures. The standard units are designed for durability with a lacquered plastic shell and nickel plated neodymium magnets placed safely inside. They fit standard test tubes and are available in sizes from 1 ml to liters. The larger custom units are proprietary designed magnetic filters (MAGicSep Process) made for efficient capture of 1-40 liters of magnetic beads from infinite volumes of complex feedstocks.

AFFINITY BEADS

Product No.: 1500

MAGicBeads mAb

Super-paramagnetic porous beads coupled with an alkali resistant protein A ligand. Optimized for purification of monoclonal antibodies directly from cell culture harvests, thus eliminating centrifugation or filtration, such as from hybridoma or CHO cells, where sanitization of beads with NaOH is desired between the uses. High bindning capacity and fast magnetic response makes the beads suitable for separation even in large-volume samples. 

  • Recover your mAb and skip centrifugation and filtration to remove cells
  • Top performance binding capacity, over 45mg IgG/ml of settled beads
  • Ability to clean the beads between the purification cycles using 0.1-0.2M NaOH
  • Stable and reusable
MAGicBeads mAb Product No.
1 ml beads 1501
5 ml beads 1502
25 ml beads 1503
1 liter beads 1504

Product No.: 1300

MAGicBeads PrtA

  • Super-paramagnetic porous beads coupled with recombinant protein A
  • Recover your mAb and skip centrifugation and filtration to remove cells
  • Top performance binding capacity, over 45mg IgG/ml of settled beads
MAGicBeads PrtA Product No.
1 ml beads 1301
5 ml beads 1302
25 ml beads 1303
1 liter beads 1304

Product No.: 1200

MAGicBeads SerA
MAGicBeads SerA are optimized for purification of antibodies from serum and ascites fluid samples and for IgG serum depletion. The beads have a binding capacity of 60 mg human or rabbit IgG per ml settled beads.

  • Purification and depletion of IgG from serum and ascites
  • Coupled with recombinant protein A.
  • Extremely high binding capacity, over 60mg IgG/ml of beads
  • Hydrophilic matrix resulting in very high specificity and low background

 

MAGicBeads SerA Product No.
1 ml beads 1201
5 ml beads 1202
25 ml beads 1203
1 liter beads 1204

data and application notes

DATA

Static binding capacity of MAGicBeads PrtA

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DATA

Comparison of clarified and non-clarified approach recovery

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APPLICATION NOTE

IP efficiency for short time pulldowns

Click on Image to read the application note

APPLICATION NOTE

Benchmark studie of protein A magnetic beads

Click on Image to read the application note

documents

MSDS

MAGicBeads mAb

 

MSDS

MAGicBeads SerA

User Instruction

MAGicBeads mAb

User Instruction

MAGicBeads SerA

MSDS

MAGicBeads PrtA

 

User Instruction

MAGicBeads PrtA

Activated beads

Product No.: 1300

MAGicBeads ACT

Surface activated magnetic agarose beads employing our patented coupling chemistry. Any ligand containing amino groups or thiols, e.g. proteins and peptides can be covalently bound to the beads using a simple process. All steps are performed at physiological conditions and room temperature, without using any hazardous reagents or organic solvents. After coupling of the ligand, the magnetic beads can be used for affinity purification and immunoprecipitation.

  • Activated magnetic beads for coupling your own ligands of choice
  • Simple coupling process at ambient temperature
  • Suitable for coupling of ligands containing sulphydryl- or amino groups
  • Gives a stable bond between bead and ligand
  • High coupling capacity, 10-30mg IgG/ml beads
  • Couple 20mg IgG/ml beads with a yield of 90%
MAGicBeads ACT Product No.
1 ml beads 1301
5 ml beads 1302
25 ml beads 1303
1 liter beads 1304

data AND Application notes

DATA

Coupling capacity studie of MAGicBeads ACT

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APPLICATION NOTE

Affinity purification of disease biomarkers

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APPLICATION NOTE

Immuno affinity purification of anti-rabbit IgG

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DOCUMENTS

MSDS

MAGicBeads ACT

User Instructions

MAGicBeads ACT

Customized magnetic beads

Product No.: 1000-custom

MAGicBeads CUSTOM

MAGic Bioprocessing are specialized in the development and production of tailor made magnetic bead products for a variety of different applications. We are able to produce customized magnetic bead products in the scale from µl up to liter of settled beads.

The parameters that can be optimized are:

  • Degree of magnetization
  • Size of beads
  • Concentration of agarose
  • Surface-functional groups
  • Ligand-coupling services

 

Please contact us for
free preliminary discussions at
custom@magicbioprocessing.com

DURABLE MAGNETIC SEPARATORS FOR LAB, PILOT AND PROCESS SCALE

The MAGicSep series of laboratory magnetic separators have been optimized for use with MAGicBeads and are ideal for most bioseparation applications. The LAB and PILOT units are designed for durability with a lacquered plastic shell and nickel plated neodymium magnets placed safely inside. The separators fit standard test tubes and are available in sizes from 1 ml to 500 ml. The combination of MAGicBeads and MAGicSep is a simple biomagnetic separation platform that requires no special training to use. The separators require very little maintenance and several batches can be processed in parallel using the same unit, making the running costs very low. The larger custom units are proprietary designed magnetic separators (MAGicSep Process) made for efficient capture of 1-40 liters of magnetic beads from infinite volumes of complex feedstocks.

 

MAGicSep unit Product no.
LAB rack 2000
LAB cube 2100
PILOT 50 2200
PILOT 500 2300
PROCESS Compact 3100
PROCESS Maxi 3400

DOCUMENTS

User Instruction

MAGicSep units

MSDS

MAGicSep units

EXPLORE OUR SCALES OF OPERATION

MAGic LAB

  • Process volumes from 50 µl to 5 ml
  • Separate from 5 µl to 200 µl of magnetic beads
  • Purify up to 6 mg proteins each cycle

MAGic PILOT

  • Process volumes from 3 ml to 500 ml per purification cycle
  • Separate from 10 µl up to 30 ml of magnetic beads
  • Purify up to 1 g proteins each cycle

MAGic PROCESS

  • Process volumes from 10 l up to 4,000 l
  • Separate from 1 l to 40 l magnetic beads
  • Recover up to 1 kg of proteins each cycle

At the research lab, small-scale processing of peptides, proteins, antibodies or cells from crude sample mixtures like serum, whole blood, ascites, food-samples or unclarified cell cultures can be performed in volumes from 100 µl to 2 ml. By using 10-200 µl of MAGicBeads together with the MAGicSep LAB cube or LAB rack enables fast, reliable and easy separation of up to 6 mg of proteins per each tube via serial or parallel-process manual configuration.

Our MAGicBeads are also compatible with robotic system such as the KingFisher™ Flex unit (Thermo Fisher) allowing the use of our beads in automatic setups for high-throughput screening.

 

data and application notes

APPLICATION NOTE

Parallel purification of scFv antibody fragments using MAGicBeads PrtA

Click on Image to read the application note

 

APPLICATION NOTE

A comparative test of MAGicBeads SerA and HiTrap® PrtA column

Click on Image to read the application note

 

MAGic LAB related products
MAGicBeads MAGicSep
MAGicBeads mAb, 1ml beads MAGicSep LAB Rack
MAGicBeads PrtA, 1ml beads MAGicSep LAB Cube
MAGicBeads SerA, 1ml beads
MAGicBeads ACT, 1ml beads

During the development phase, medium-scale processing of peptides, proteins or antibodies from clarified- or unclarified cell-culture mixtures is performed in sample volumes from 5 ml up to a few liters. The use of the MAGicSep PILOT 50 or MAGicSep PILOT 500 together with 200µl – 30ml of settled MAGicBeads offers a durable, convenient and fast separation platform of up to gram quantities of proteins per each bottle via serial or parallel-process manual configuration.

By combining the different MAGicSep units, low expressed target peptides and proteins can be captured from a large volume and then washed and eluted in a smaller sized vessel, thus achieving a significant product concentration.

data and application notes

APPLICATION NOTE

Time study of parallel mAb purification from CHO-cell broth at 1ml MAGicBeads PrtA scale

Click on Image to read the application note

MAGic PILOT related products
MAGicBeads MAGicSep

MAGicBeads mAb, 5 and 25ml beads

MAGicSep PILOT 50
MAGicBeads PrtA, 5 and 25ml beads MAGicSep PILOT 500
MAGicBeads SerA, 5 and 25ml beads
MAGicBeads ACT, 5 and 25ml beads

During the production phase of biopharmaceuticals, large-scale recovery of the product from an unclarified cell-culture is performed in a one step clarification and purification by using the propietary designed MAGicSep PROCESS units together with the affinity-coupled MAGicBeads. The magnetic filter separators are capable to capture up to 40 liters of MAGicBeads at high flow rates making them suitable to process up to kilo grams of proteins or antibodies per purification cycle.

By utilizing the magnetic-based capture approach, the biopharmaceutical can be recovered directly from the cell-containing culture with the same or better quality as for a conventional 2-step clarification and purification mode using depth filters and column chromatography.

  • Process scale recovery of biologics
  • High flow rates, 100-800 liter/h
  • Pharmaceutical standard
  • No column-clogging issues
  • Automated
  • 24/7 operation
  • Captures 1-40 liters of MAGicBeads per cycle
  • Process 10-4,000 liters of culture volumes
  • Recover kgs of mAbs per cycle

PROCESS PARAMETERS

PROCESS Compact PROCESS Medio PROCESS Maxi

Product No.:

3100

Product No.:

3300

Product No.:

3400

Bead capacity [liter]

2

Bead capacity [liter]

12

Bead capacity [liter]

40

Flow rate [l/h]

100

Flow rate [l/h]

400

Flow rate [l/h]

800

Feedstock volume [l]

10-100

Feedstock volume [l]

60-600

Feedstock volume [l]

200-4,000

Intrested in magnetic separators for process scale?

Please contact us at

process@magicbioprocessing.com

data

POSTER

Magnetic bead purification of mAb from cell broth

Click on Image to view the poster

 

Scientific paper

Magnetic bead capture and recovery of mAb from crude cell-broth

Click on Image to read the full paper