Antibody capture process based on magnetic beads from very high cell density suspensions

The paper provides a proof-of-concept demonstrating magnetic bead separation of monoclonal antibodies from non-clarified cell suspension at very high cell density. The objective was to apply the technology downwards of intensified fed-batch process using a UF-HF filter-based perfusion. The culture process is known to be highly challenging for cell clarification, and represents an excellent challenge to demonstrate the potential of magnetic separation, including a significant simplification of the operational steps. The paper aimed at maintaining a high product yield over the mAb capture from non‐clarified high cell density suspension, with purity comparable or improved compared to legacy approach of cell clarification followed by column chromatography.

The work was performed in collaboration with CETEG at KTH within the AdBIOPRO competence center.